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Faculty
of Science/Biological Sciences
ELECTRON
MICROSCOPY UNIT
History
The department first introduced
Electron Microscopy in 1984 when the Government of Japan presented
a JEOL JEM 100 CXII STEM equipped with X-ray Microanalysis attachment
and other sample preparation equipment. It was at that time the
most up-to-date facility in Singapore and it served the whole of
the Science Faculty and other institutes, such as SGH. It had
analysed a wide range of biological and physical samples from
unicellular organisms to tissues of both botanical and zoological
nature and from nanoparticles to geological samples. It continues
to be used by research personnel from many Faculties and Departments
of NUS and industry.
Currently the Unit has:
TEMs:
-
JEOL JEM 3010 HRTEM & EDX
(300KV LaB6) with Cooling/Heating sample Holder
For conventional HRTEM imaging, Electron Diffraction
Pattern, Elemental X-ray micro-analysis
-
JEOL JEM 2010F HRTEM (200KV
Field Emission Gun) with Cryo-sample Holder
For conventional HRTEM imaging, Electron Diffraction Pattern
-
PHILIPS CM10 TEM (100KV W)
For conventional transmitted electron imaging.
SEM:
Sample preparation Equipment
For TLM & TEM
Tissue Processors (LM & TEM)
Embedding ovens (normal & vacuum)
Microtomes (Wax & Resin; Conventional & Cryo.)
Cutter, Grinder, Dimpler & Ion Beam Miller
Vacuum Evaporator
For SEM
Critical Point Dryer
Ion Sputter for gold coating
Vacuum Evaporator for carbon coating
Rules and Regulations
A form needs to be filled out by
the applicant and signed by his/her supervisor or PI before bookings
can be made for training/use of instuments, EMs and darkroom. Each
user is allowed to book only one session (9 am-1 pm; 2 pm- 6 pm
Friday 5.30pm) in advance. Any cancellation of session booked must
be informed as early as possible so that others can use the slot.
Usage of all equipment at any time must be recorded in their respective
log/record books placed with the equipment.
List of Charges
All training in sample preparation
and demonstration of equipment and EMs are provided free of charge
but consumables used will be charged. Electron Microscope usage
will also be charged per hour as per attached on a bi-annual basis:
CHARGES FOR
EM FACILITY USERS
| 1 |
TEM sheet film (inclusive of
envelop and chemicals for developing) |
$2.00 |
| 1 |
Critical Point Drying |
$5.00 |
| 1 |
Gold Coating |
$20.00 |
| 1 |
Carbon Coating |
$5.00 |
| 1 |
session (4 hrs) of Physical
Sample Preparation |
$10.00 |
| 1 |
vial Glutaraldehyde |
$20.00 |
| 1 |
vial Osmium tetra-oxide |
$20.00 |
| 1 |
sample of EDS (X-ray micro-analysis) |
$10.00 |
| 1 |
hour of Freeze Substitution |
$5.00 |
| 1 |
hour of Microtomy (Wax / Resin) |
$5.00 |
| 1 |
hour of Cryo-ultramicrotomy |
$15.00 |
| 1 |
hour of Cryo-stat sectioning |
$5.00 |
| 1 |
hour of Cryo-fixation (with
KF80) |
$5.00 |
| 1 |
Embedding/Use of Tissue Processor |
$10.00 |
| |
|
|
| |
|
SEM/TEM |
HRTEM |
| 1 |
hour usage of Electron Microscopy: |
Outside NUS |
$100.00 |
$200.00 |
| |
|
Within NUS |
$ 20.00 |
$50.00 |
Charged Service and Training is
also provided to Industries depending on sample.
Persons to Contact:
Unit Manager:
Mdm Loy Gek Luan (Ext. 2718)
Lab Staff:
Mdm.Loy Gek Luan (Lab Technologist
I, Ext. 2718)
Mr. Chong Ping Lee (Lab Technologist II, Ext 6321)
Application
Form |
