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Assistant Professor HUANG DEJIAN
B.S., 1987, Fujian Teachers University; M.S., 1990, Chinese Academy of Sciences; Ph.D., 1999, Indiana University Bloomington; Post Doctoral Fellow, 1999-2001, Massachusetts Institute of Technology.
Contact Information:
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Department of Chemistry, NUS
3 Science Drive 3
Singapore 117543
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Office: S3-06-05
Tel: (65)-6516-8821
Fax: (65)-6775-7895
Email: chmhdj@nus.edu.sg |
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Research Interests
Antioxidation / oxidation and its role in yin / yang balance in Chinese Traditional Medicine (TCM)
The balance between antioxidation and oxidation is believed to be critical for our health. Diseases would occur if the balance is lost, primarily, due to oxidative stress, which results from excessive and unwanted oxidative damage of bio-molecules by reactive oxidants. Ancient, yet more and more widely being practiced, traditional Chinese medicine (TCM) is based on yin-yang balance theory, which states that the imbalance of yin-yang was the cause of all diseases. The yin and yang, however, does not have any concrete physical meaning within the modern scientific scope and thus there is no equivalent term in western medicine to express this dichotomy. My aim is to unveil the molecular mechanisms of yin-yang in TCM and its relationship with antioxidation-oxidation. To reach this goal, we can investigate from several different angles and one of them is studying Chinese herbal medicine. One important group of herbal medicine is antipyretic herbs. In TCM, antipyretic herbs are mainly administered in clearing excessive heat or "fire", which is often the symptom of excessive "yang", or lack of "yin". At current stage we will investigate the antioxidant capacity of representative herbs, elucidate the chemical structures, and probe their reaction mechanisms with reactive oxygen species relevant to oxidative stress. Students will have the opportunity to learn antioxidants, oxidative stress and their role in disease. In addition, through the laboratory practices, students will learn a wide range of experimental techniques such as, high throughput screening assays using micro-plate fluorescence readers, high performance liquid chromatograph (HPLC), LC-MS, multi-dimensional NMR, IR, and chemical separation techniques.
Antioxidants in food and cosmetic emulsion systems
Antioxidants not only have health benefits but also critical in
extending shelf life of food products, which often contain mixtures
of oil and water phases. Currently there is no convenient assay
to evaluate antioxidant capacity in a food system, particularly
in emulsions and foams, which are heterogeneous and the reaction
cannot be easily monitored in real time. Conventional chemical analysis
on these matrixes requires tedious sample treatments. Thus the efficiency
is fairly low and the results are not quantitative. Our aim is to
develop a high throughput assay that can be used to monitor oxidation
progress of an emulsion system in real time taking advantage of
oxygen sensor coated micro-plate. The capacity of antioxidants in
emulsion can thus be quantified. The assay will be a valuable tool
for identifying better antioxidants for food preservations and cosmetic
product applications.
Respective Publications
Patents
03. Dejian Huang, Boxin Ou, “A method in quantitation of oxidants
in smoke”, U. S. patent pending Number: 10/328666 (filing date:
Dec. 15, 2002).
02 . Boxin Ou, Dejian Huang, “ Fluorometric Assays of Antioxidants
on Peroxyl Radical Absorption Capacity”, U. S. patent pending,
(filing date: November 15, 2001)
01 . Jackie Y. Ying, John S. Lettow, Dejian Huang, Christian Mehnert,
“Surface-confined Catalytic Compositions”, U.S. Patent
Application No. 60/150,672 (filing date: Aug. 25, 2000, approved,
Dec. 03, 2002).
Publications
 Dejian Huang*, Boxin Ou,
Ronald L. Prior, “the Chemistry behind antioxidant assays”
(Review) J. Agri. Food Chem.In press (*corresponding author).
Prior, R. L., Hoang, H.,
Gu, L. W., Wu, X. L., Bacchiocca, M., Howard, L., Hampsch-Woodill.
M., Huang, D. J., Ou, B. Jacob, R. Assays for hydrophilic and lipophilic
antioxidant capacity (oxygen radical absorbance capacity (ORAC(FL)))
of plasma and other biological and food samples J. Agri. Food Chem
2003, 51 (11): 3273-3279.
Naguib, Y. M. A.; Hari, S.
P.; Passwater, R.; Huang, D. J. Antioxidant activities of natural
vitamin E formulations J. NUTRITIONAL SCIENCE AND VITAMINOLOGY 2003,
49 (4): 217-220.
Boxin Ou, Dejian Huang*,
Maureen Hampsch-Woodill , Judith A. Flanagan, “When East Meets
West: the relationship between yin/yang and antioxidation/oxidation”
FASEB J. 2003 17(2), 127-129.
Boxin Ou, Dejian Huang, Maureen
Hampsch-Woodill, Judith A. Flanagan, Elisabeth K. Deemer, “
Analysis of Antioxidant Activities of Common Vegetables Employing
Oxygen Radical Absorbance Capacity (ORAC) and Ferric Reducing Antioxidant
Power (FRAP) Assays: A Comparative Study” J. Agric. Food Chem.
2002 ; 50 (11); 3122-3128.
Dejian Huang, Boxin Ou, Maureen
Hampsch-Woodill, Judith Flanagan, Elizabeth K. Deemer; “Development
and Validation of Oxygen Radical Absorbance Capacity for Lipophilic
Antioxidants Using Randomly Methylated ?- Cyclodextrin as the Solubility
Enhancer” J. Agri. Food Chem. 2002 ; 50 (7); 1815-1821.
Boxin Ou, Maureen Hampsch-Woodill,
Judith Flanagan, Elizabeth K. Deemer, and Ronald L. Prior, Dejian
Huang* “A Novel Fluorometric Assay for Hydroxyl Radical Preventing
Capacity Using Fluorescein as the Probe”. J. Agri. Food Chem.
2002 ; 50 (10); 2772-2777.
Dejian Huang, Boxin Ou, Maureen
Hampsch-Woodill, Judith Flanagan, “Full Automation of Oxygen
Radical Absorbance Capacity Assay on 96-Well Plate Fluorescence
Reader Coupled with Multi-Channel Liquid Handling System”.
J. Agric. Food Chem. 2002 ; 50 (16); 4437-4444.
Yandulov , DV, Huang, DJ,
Huffman, JC, Caulton, KG, Structural distortions in mer-M(H)(3)(NO)L-2
(M =
Ru , Os) and their influence on intramolecular fluxionality and
quantum exchange coupling, Inorg. Chem, 2000, 39, 1919-32.
Ferrando, G.; Coalter, J.
N.; Gérard, H.; Huang, D.; Eisenstein, O.; Caulton, K. G.
Facile C(sp 2)/O 2CR bond cleavage by Ru or Os, New J. Chem. 2003,
(10), 1451 - 1462
Dejian Huang, Patrick R.
Koren, Kirsten Folting, Ernest R. Davidson, and Kenneth G. Caulton;
“Facile and Reversible Cleavage of C-F Bonds. Contrasting Thermodynamic
Selectivity for Ru-CF 2H vs F-Os=CFH”, J. Am. Chem. Soc.; 2000;
122(37); 8916-8931.
Dejian Huang, John C. Bollinger,
William E. Streib, Kirsten Folting, Victor Young, Jr., Odile Eisenstein,
and Kenneth G. Caulton; “A 14-Electron Ruthenium(II) Hydride,
[ RuH(CO)(P tBu 2Me) 2]BAr' 4 ( Ar' = 3,5-(C 6H 3)(CF 3) 2): Synthesis,
Structure, and Reactivity toward Alkenes and Oxygen Ligands”,
Organometallics;2000; 19(12); 2281-2290.
Dejian Huang, Kirsten Folting,
and Kenneth G. Caulton; “ Silyl Migration of Me 3SiCCPh Coordinated
to [ RuH(CO)(P tBu 2Me) 2]BAr' 4 Can Be Reversed: Synthesis and
Structure of [ Ru(CH=C(SiMe 3)(Ph))(CO)(P tBu 2Me) 2]BAr' 4 ”,
J. Am. Chem. Soc.; 1999; 121(44); 10318-10322.
Dejian Huang, William E.
Streib, John C. Bollinger, Kenneth G. Caulton,, Rainer F. Winter,,
and Thomas Scheiring; “14-Electron Four-Coordinate Ru(II) Carbyl
Complexes and Their Five-Coordinate Precursors: Synthesis, Double
Agostic Interactions, and Reactivity”, J. Am. Chem. Soc.; 1999;
121(35); 8087-8097.
Masamichi Ogasawara, Dejian
Huang, William E. Streib, John C. Huffman, Nuria Gallego-Planas,
Feliu Maseras, Odile Eisenstein, and Kenneth G. Caulton; “
RuX(CO)(NO)L 2 and Ru(CO)(NO)L 2 +: Ru(0) or Ru(II) or In Between?”,
J. Am. Chem. Soc.; 1997; 119(37); 8642-8651.
Dejian Huang, John C. Huffman,
John C. Bollinger, Odile Eisenstein,* and Kenneth G. Caulton; “The
First ? 2-CH 2Cl 2 Adduct of Ru(II):[ RuH(? 2-CH 2Cl 2)(CO)(P tBu
2Me) 2][BAr' 4] ( Ar' = 3,5-C 6H 3(CF 3) 2) and Its RuH(CO)(P tBu
2Me) 2 + Precursor”, J. Am. Chem. Soc.; 1997; 119(31); 7398-7399.
Dejian Huang and Kenneth
G. Caulton; “New Entries to and New Reactions of Fluorocarbon
Ligands”, J. Am. Chem. Soc.; 1997; 119(13); 3185-3186.
Huang, D. J. Streib, W. E.Eisenstein,O,
Caulton, K. G. [ Ru(Ph)(CO)(PtBu2Me)(2)](+): A unique 14-electron
Ru-II complex with two agostic interactions Angew Chem.Int. Ed.1997,
36, 2004-06.
Dejian Huang, William E.
Streib, Odile Eisenstein, and Kenneth G. Caulton; “16-Electron
Ruthenium(0) Complexes Containing the Ru(NO)L 2 + Substructure:
Planar RuCH 3(NO)L 2 vs Sawhorse [ Ru(NO)(C=C(SiMe 3) 2)L 2] +”,
Organometallics; 2000; 19(10), 1967-1972.
Dejian Huang, Hélène
Gérard, Eric Clot, Victor Young, Jr., William E. Streib,
Odile Eisenstein, and Kenneth G. Caulton; “L' = CO vs Cl- Transposition:
Remarkable Consequences for the Product of (L')- Ru(L) 2-(H) and
Vinyl Ether”, Organometallics; 1999; 18(26); 5441-5443.
Dejian Huang, Montse Oliván,
Odile Eisenstein, John C. Huffman, Kenneth G. Caulton; “CO-Induced
C(sp 2)/C(sp) Coupling on Ru and Os: A Comparative Study”,
Organometallics; 1998; 17(21); 4700-4706.
Dejian Huang, John C. Bollinger,
Kenneth G. Caulton; “Oxidative Addition of a Si-C(sp) Bond
to Ruthenium: Synthesis and Reactivity of Ru(SiMe 3)(CCSiMe 3)(CO)(P
tBu 2Me) 2”, Organometallics;1997;16(3); 292-293.
Dmitry V. Yandulov, Dejian
Huang, John C. Huffman, and Kenneth G. Caulton; Structural Distortions
in mer-M(H) 3(NO)L 2 (M = Ru, Os) and Their Influence on Intramolecular
Fluxionality and Quantum Exchange Coupling”, Inorg. Chem.;
2000; 39(9); 1919-1932.
Dejian Huang, Kirsten Folting,
Kenneth G. Caulton, “Reactivity of RuCl 2(CO)(P tBu 2Me) 2
towards H 2 and Nucleophiles: Aggregation Triggered by Protonation
and Phosphine Loss”, Inorg. Chem. 1996, 35, 7035.
Dejian Huang, Jun Yang, Wen
Wang, Liangfu Zhang; “ Cholesteric Metallomesogens Containing
Optically Active Metal- Tricarbonyl Moieties”, Mol. Cryst.
Liq. Cryst, 1996, 281, 43.
Jun Yang, Dejian Huang, Liangfu
Zhang; Wenjie Guang; “Metal Tricarbonyl Group Incorporated
Mesogens”, Mol. Cryst. Liq. Cryst.1996, 281, 51.
Liangfu Zhang, Wen Wang,
Dejian Huang, Jun Yang, Guangnian Li; “New Properties of Organometallic
Ferroelectric Liquid Crystals”, Huaxue Tongbao, 1995, 7.
Dejian Huang, Liangfu Zhang,
Nuyun Xiong, “ Organometallic Ferroelectric Liquid Crystals
II. Synthesis, Characterization and Mesogenic Properties of Amino
Acidate Chelated Palladium(II) Complexes of Azine, Azo, and Imine”,
Mol. Cryst. Liq. Cryst.1993, 231, 191.
Liangfu Zhang, Dejian Huang,
Nuyun Xiong; “ Organometallic Ferroelectric Liquid Crystals
III. Synthesis and Liquid Crystal Properties of ortho Palladated
Binuclear Complexes of Azine, Azo, and Imine Derivatives”,
Mol. Cryst. Liq. Cryst. 1993, 237, 285.
Liangfu Zhang, Jun Yang,
Dejian Huang, Guangnian Li, “Synthesis and Molecular Structure
of Phosphine- Sulfur Hybrid Atom Ligands and their Palladium Complexes
with the Studies on the C-H Bond Activation and Photochemical Carbonylation
Reaction of Benzene”, Science in China(Series B) 1993, 36,
325.
Jun Yang, Dejian Huang, Guangnian
Li; “Synthesis, Structure and C-H Bond Activation of Platinum(II)
Complexes Containing Phosphorus and Sulfur Hybrid Ligands”,
Acta Chimica Sinica1993, 35, 1150.
Dejian Huang, Linhui Tong;
“The Spectroscopic Study of the Interactions of 5-Deazaflavin
and Metal Ions”, Chinese Science Bulletin1992, 224.
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